Cellular damage induced by cadmium and mercury in Medicago sativa

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Cellular damage induced by cadmium and mercury in Medicago sativa.

Alfalfa (Medicago sativa) plantlets were exposed to Cd or Hg to study the kinetics of diverse stress indexes. In the so-called beaker-size hydroponic system, plantlets were grown in 30 microM of Cd or Hg for 7 d. Oxidative stress took place and increased over time, a linear response being observed with Cd but not with Hg. To improve the sensitivity of the stress assays used, a micro-assay syste...

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Cadmium-Induced Hydrogen Sulfide Synthesis Is Involved in Cadmium Tolerance in Medicago sativa by Reestablishment of Reduced (Homo)glutathione and Reactive Oxygen Species Homeostases

Until now, physiological mechanisms and downstream targets responsible for the cadmium (Cd) tolerance mediated by endogenous hydrogen sulfide (H2S) have been elusive. To address this gap, a combination of pharmacological, histochemical, biochemical and molecular approaches was applied. The perturbation of reduced (homo)glutathione homeostasis and increased H2S production as well as the activati...

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Rhizophagus irregularis regulates antioxidant activity and gene expression under cadmium toxicity in Medicago sativa

Cadmium (Cd) is a phytotoxic heavy metal (HM) that can induce generation of reactive oxygen species (ROS). Arbuscular mycorrhizal fungi (AMF) are considered as bio-ameliorators that help to mitigate HM-derived oxidative stress. The objective of this study was to assess AM fungus Rhizophagus irregularis on changes in enzymatic activity and transcription of antioxidants of Medicago sativa to Cd s...

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Arbuscular Mycorrhizal Colonization Alters Subcellular Distribution and Chemical Forms of Cadmium in Medicago sativa L. and Resists Cadmium Toxicity

Some plants can tolerate and even detoxify soils contaminated with heavy metals. This detoxification ability may depend on what chemical forms of metals are taken up by plants and how the plants distribute the toxins in their tissues. This, in turn, may have an important impact on phytoremediation. We investigated the impact of arbuscular mycorrhizal (AM) fungus, Glomus intraradices, on the sub...

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Haem oxygenase-1 is involved in salicylic acid-induced alleviation of oxidative stress due to cadmium stress in Medicago sativa

This work examines the involvement of haem oxygenase-1 (HO-1) in salicylic acid (SA)-induced alleviation of oxidative stress as a result of cadmium (Cd) stress in alfalfa (Medicago sativa L.) seedling roots. CdCl(2) exposure caused severe growth inhibition and Cd accumulation, which were potentiated by pre-treatment with zinc protoporphyrin (ZnPPIX), a potent HO-1 inhibitor. Pre-treatment of pl...

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ژورنال

عنوان ژورنال: Journal of Experimental Botany

سال: 2005

ISSN: 1460-2431,0022-0957

DOI: 10.1093/jxb/eri223